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Application of Deep Sequencing in Phage Display

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2738))

Abstract

This chapter describes the workflow to implement deep sequencing into standard phage display experiments on protein libraries. By harvesting the power of high throughput of these techniques, it allows for comprehensive analysis of the naïve library and library evolution in response to selection by ligand binding. The mutagenized target region of the protein variants encoded by the phage pool is analyzed by Illumina paired-end sequencing. Sequence data are processed to extract selection-enriched amino acid motifs. In addition, a complementary long-read sequencing approach is proposed enabling the monitoring of display vector stability.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Molecular and Structural Biology, KU Leuven, Leuven, Belgium

    Vincent Van Deuren, Sander Plessers & Johan Robben

  2. Animal and Human Health Engineering (A2H), Leuven (Arenberg), KU Leuven, Leuven, Belgium

    Rob Lavigne

Authors
  1. Vincent Van Deuren
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  2. Sander Plessers
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  3. Rob Lavigne
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  4. Johan Robben
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Corresponding author

Correspondence to Johan Robben .

Editor information

Editors and Affiliations

  1. School of Veterinary Medicine, Texas Tech University, Amarillo, TX, USA

    Ebenezer Tumban

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Van Deuren, V., Plessers, S., Lavigne, R., Robben, J. (2024). Application of Deep Sequencing in Phage Display. In: Tumban, E. (eds) Bacteriophages. Methods in Molecular Biology, vol 2738. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3549-0_20

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  • DOI: https://doi.org/10.1007/978-1-0716-3549-0_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3548-3

  • Online ISBN: 978-1-0716-3549-0

  • eBook Packages: Springer Protocols

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