Abstract
The Cannabis plant has been smoked for medicinal and recreational purposes for thousands of years. Tetrahydrocannabinol (THC) is the most well-known psychoactive cannabinoid, and the properties of other cannabinoids are becoming better understood. Due to increased exposure, hospitals and clinics will need access to rapid and accurate THC testing procedures to better inform patients and improve care. A rapid and reliable HPLC-MS/MS method was developed for the quantitative assessment of two THC metabolites (THC-COOH and THC-COO(Gluc)). The chromatographic separation was done using a short (50 × 4.6 mm) phenyl-hexyl column with positive ESI mass spectrometry analysis. To reduce interferences and improve quantitation, the assay was run using multiple reaction monitoring mode. The method was shown to be precise (<15% CV with-in day & between day), accurate (±20%) and linear (>R2 0.99) within the range of 25–8000 ng/mL.
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References
Atakan Z (2012) Cannabis, a complex plant: different compounds and different effects on individuals. Ther Adv Psychopharmacol 2:241–254
Andre CM, Hausman JF, Guerriero G (2016) Cannabis sativa: the plant of the thousand and one molecules. Front Plant Sci 7:19
Page RL, Allen LA, Kloner RA et al (2020) Medical marijuana, recreational cannabis, and cardiovascular health: a scientific statement from the American Heart Association. Circulation 142:E131–E152
Pertwee RG (2006) Cannabinoid pharmacology: the first 66 years. Br J Pharmacol 147:163–171
Abrams DI (2018) The therapeutic effects of Cannabis and cannabinoids: an update from the National Academies of Sciences, Engineering and Medicine report. Eur J Intern Med 49:7–11
Huestis MA (2001) Cannabis. In: Kwong TC, Magnani B, Rosano TG, Shaw LM (eds) The clinical toxicology laboratory: contemporary practice of poisoning evaluation, 2nd edn. AACC Press, pp 119–137
Ujváry I, Hanuš L (2016) Human metabolites of cannabidiol: a review on their formation, biological activity, and relevance in therapy. Cannabis Cannabinoid Res 1:90–101
Kraemer M, Broecker S, Diehl BWK et al (2019) Palmitic acid ester of tetrahydrocannabinol (THC) and palmitic acid diester of 11-hydroxy-THC—unsuccessful search for additional THC metabolites in human body fluids and tissues. Forensic Sci Int 294:86–95
Agostini M, Renzoni C, Pierini E et al (2019) Rapid, hydrolysis-free, dilute-and-shoot method for the determination of buprenorphine, norbuprenorphine and their glucuronides in urine samples using UHPLC-MS/MS. J Pharm Biomed Anal 166:236–243
Young B, Zhang YV (2022) A rapid dilute-and-shoot LC-MS/MS method for quantifying THC-COOH and THC-COO(Gluc) in urine. J Chromatogr B 1211:123495
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Young, B., Zhang, Y.V. (2024). A Direct Dilute-and-Shoot HPLC-MS/MS Method for Tetrahydrocannabinol (THC) Metabolites. In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 2737. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3541-4_40
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DOI: https://doi.org/10.1007/978-1-0716-3541-4_40
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