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Reverse Genetics Systems for Filoviruses

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Reverse Genetics of RNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2733))

Abstract

Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable. Here, we describe the generation of recombinant filoviruses from cDNA.

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Acknowledgments

This work was supported by the Friedrich-Loeffler-Institut through intramural funding and the Deutsche Forschungsgemeinschaft (DFG; grant number 452208680). The authors further are grateful to Allison Groseth (Friedrich-Loeffler-Institut) for critical reading of this manuscript.

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  1. Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany

    Bianca S. Bodmer & Thomas Hoenen

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  1. Bianca S. Bodmer
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  2. Thomas Hoenen
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Correspondence to Thomas Hoenen .

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Editors and Affiliations

  1. College of Veterinary Medicine, University of Georgia, Athens, GA, USA

    Daniel R. Pérez

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Bodmer, B.S., Hoenen, T. (2024). Reverse Genetics Systems for Filoviruses. In: Pérez, D.R. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 2733. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3533-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3533-9_1

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