Abstract
Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.
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Terrance, M. et al. (2024). Primary Human Nasal Epithelial Cell Culture. In: Mennella, V. (eds) Cilia. Methods in Molecular Biology, vol 2725. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3507-0_13
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DOI: https://doi.org/10.1007/978-1-0716-3507-0_13
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3507-0
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