Abstract
Normal fetal growth and placental development depend on active angiogenesis occurring at the fetomaternal interface throughout pregnancy. Nevertheless, reliable in vivo methods to assess placental angiogenesis are still missing. Here, we describe a quantitative and noninvasive in vivo method to specifically measure placental neovascularization in the gravid mouse. This method uses a technique based on the measurement of a fluorescent molecule Angiostamp700 that targets the alpha v beta 3 (αvβ3) integrin, a protein that is highly expressed by endothelial cells during the neovascularization and by trophoblast cells during invasion of the maternal decidua. Due to this noninvasive method, quantification of the fetomaternal angiogenic activity and information regarding the outcome of pregnancy are now possible.
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Acknowledgments
We acknowledge the following sources of funding: INSERM (U1292), University Grenoble-Alpes Fourier ANR-17-EURE-0003, Commissariat à l’Energie Atomique (IRIG/DS/Biosanté) and Institute for Advanced Biosciences, INSERM-UGA U1209, CNRS UMR 5309. The Optimal imaging platform is supported by France Life Imaging (French program “Investissement d’Avenir” grant; “Infrastructure d’avenir en Biologie Santé”, ANR-11-INBS-0006) and the IBISA French consortium “Infrastructures en Biologie Santé et Agronomie”. Imaging systems used in this study were purchased thanks to France Life Imaging (French program “Investissement d’Avenir” grant; “Infrastructure d’avenir en Biologie Sante”, ANR-11-INBS-0006) and the Contrat Plan Etat-Région Auvergne-Rhone-Alpes.
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Josserand, V. et al. (2024). RGD-Based Fluorescence to Assess Placental Angiogenesis. In: Raha, S. (eds) Trophoblasts. Methods in Molecular Biology, vol 2728. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3495-0_11
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DOI: https://doi.org/10.1007/978-1-0716-3495-0_11
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