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Pseudomonas aeruginosa Soluble Pyocins as Antibacterial Weapons

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Pseudomonas aeruginosa

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2721))

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen causing nosocomial infections and associated with lung infections in cystic fibrosis (CF) patients (Lyczak et al., Microbes Infect 2:1051–1060, 2000). Multiple drug-resistant P. aeruginosa strains pose a serious problem because of antibiotic treatment failure. There is therefore a need for alternative anti-Pseudomonas molecules. Soluble pyocins (S-pyocins) are bacteriocins produced by P. aeruginosa strains that kill sensitive strains of the same species. These bacteriocins and their immunity gene are easily cloned and expressed in E. coli and their activity spectrum against different P. aeruginosa strains can be tested. In this chapter, we describe the procedures for cloning, expression, and sensitivity testing of two different S-pyocins. We also describe how to identify their receptor binding domain in sensitive strains, how to construct chimeric pyocins with extended activity spectra, and how to identify new pyocins in genomes by multiplex PCR.

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Author information

Authors and Affiliations

  1. Vrije Universiteit Brussel, Microbiology Group, Brussels, Belgium

    Pierre Cornelis & Jozef Dingemans

  2. Institut de Génétique et de Développement de Rennes (IGDR), CNRS UMR 6290, Université de Rennes, Rennes, France

    Christine Baysse

Authors
  1. Pierre Cornelis
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  2. Jozef Dingemans
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  3. Christine Baysse
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Corresponding author

Correspondence to Pierre Cornelis .

Editor information

Editors and Affiliations

  1. Department of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy

    Giovanni Bertoni

  2. Department of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy

    Silvia Ferrara

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cornelis, P., Dingemans, J., Baysse, C. (2024). Pseudomonas aeruginosa Soluble Pyocins as Antibacterial Weapons. In: Bertoni, G., Ferrara, S. (eds) Pseudomonas aeruginosa. Methods in Molecular Biology, vol 2721. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3473-8_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3473-8_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3472-1

  • Online ISBN: 978-1-0716-3473-8

  • eBook Packages: Springer Protocols

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