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Targeted Profiling of Protein Phosphorylation in Plants

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Mass Spectrometry-Based Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2718))

Abstract

Proteins are crucial for controlling different cellular processes by perceiving and converting external environmental cues into cellular responses. Therefore, regulation of protein activities is pivotal for the development and survival of an organism. This is often mediated by posttranslational modifications, which usually are carried out on specific residues of a target protein by a “writer” protein. The (reversible) modifications of different residues may lead to different signaling outputs. In the case of protein phosphorylation, one of the most common posttranslational modifications, this writer protein is a protein kinase. In this chapter, we report a comprehensive and versatile workflow to identify the phosphorylation profile of a target protein in plants from a putative kinase-target pair by combining an in planta phosphorylation assay and mass spectrometry analysis.

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Authors and Affiliations

  1. Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium

    Xiangyu Xu, Ive De Smet & Lam Dai Vu

  2. VIB Center for Plant Systems Biology, Ghent, Belgium

    Xiangyu Xu, Ive De Smet & Lam Dai Vu

  3. VIB-UGent Center for Medical Biotechnology, Ghent, Belgium

    Kris Gevaert & Lam Dai Vu

  4. Department of Biomolecular Medicine, Ghent University, Ghent, Belgium

    Kris Gevaert & Lam Dai Vu

Authors
  1. Xiangyu Xu
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  2. Kris Gevaert
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  3. Ive De Smet
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  4. Lam Dai Vu
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Corresponding author

Correspondence to Ive De Smet .

Editor information

Editors and Affiliations

  1. VIB-UGent Center for Medical Biotechnology, Ghent, Belgium

    Kris Gevaert

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Xu, X., Gevaert, K., De Smet, I., Vu, L.D. (2023). Targeted Profiling of Protein Phosphorylation in Plants. In: Gevaert, K. (eds) Mass Spectrometry-Based Proteomics. Methods in Molecular Biology, vol 2718. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3457-8_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3457-8_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3456-1

  • Online ISBN: 978-1-0716-3457-8

  • eBook Packages: Springer Protocols

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