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Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA)

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Food Allergens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2717))

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for food allergen detection and quantification. Validating ELISA protocols is important for both assay developers and end users as it ensures method reliability. This chapter describes the protocols for validating the sensitivity, specificity, precision, accuracy, robustness, and ruggedness of an ELISA. Example procedures are also provided for sample preparation, allergen extraction, and ELISA operation.

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Acknowledgments

Our late professor, Dr. Shridhar K. Sathe, is acknowledged for his guidance.

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Authors and Affiliations

  1. School of Exercise and Nutritional Sciences, San Diego State University, San Diego, CA, USA

    Changqi Liu

  2. Fujian Hwa Nan Women’s College, Fuzhou, Fujian, China

    Mengna Su

  3. PerkinElmer Inc., Austin, TX, USA

    Valerie D. Champ

  4. General Mills, Inc., Minneapolis, MN, USA

    Sahil Gupta

Authors
  1. Changqi Liu
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  2. Mengna Su
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  3. Valerie D. Champ
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  4. Sahil Gupta
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Corresponding author

Correspondence to Changqi Liu .

Editor information

Editors and Affiliations

  1. Department of Allergy, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain

    Beatriz Cabanillas

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Liu, C., Su, M., Champ, V.D., Gupta, S. (2024). Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA). In: Cabanillas, B. (eds) Food Allergens. Methods in Molecular Biology, vol 2717. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3453-0_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3453-0_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3452-3

  • Online ISBN: 978-1-0716-3453-0

  • eBook Packages: Springer Protocols

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