Abstract
Ferroptosis is a regulated form of cell death characterized by the accumulation of oxidized phospholipids, particularly oxidized phosphatidylethanolamines (PE), which serve as important biomarkers in the progression of various diseases. To facilitate the comprehensive investigation of ferroptosis in biological systems, we present a robust and versatile untargeted redox phospholipidomics method employing normal-phase liquid chromatography-mass spectrometry (LC-MS). This high-throughput technique enables the identification and quantification of dozens of oxidized phospholipid species in a single run, providing valuable insights into the molecular mechanisms underlying ferroptosis. It has been successfully applied to diverse biological samples, including human patients, animals, and cell cultures, and offers a powerful tool for investigating the roles of oxidized phospholipids in the development and progression of various diseases.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Sun, WY., Wang, R., He, RR. (2023). LC-MS-Based Redox Phosphoipidomics Analysis in Ferroptosis. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_8
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DOI: https://doi.org/10.1007/978-1-0716-3433-2_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3432-5
Online ISBN: 978-1-0716-3433-2
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