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Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence

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Ferroptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2712))

Abstract

Ferroptosis is a type of regulated cell death driven by oxidative damage, characterized by iron overload and lipid peroxidation, and regulated by a network of distinct molecules and organelles. Impaired ferroptotic response is implicated in multiple physiological and pathological processes, including tumorigenesis, neurodegeneration, and ischemia-reperfusion damage. Classical techniques of immunohistochemistry (IHC) and immunofluorescence (IF) can be employed to exhibit antigen expression and location in tissues observed with microscopy, making them powerful tools in studying the ferroptosis process. In this chapter, we introduce commonly used protocols and summarize typical markers used in IHC and IF to monitor ferroptosis.

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Authors and Affiliations

  1. Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Xiao Zhong & Ruochan Chen

  2. Hunan Key Laboratory of Viral Hepatitis, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Xiao Zhong & Ruochan Chen

Authors
  1. Xiao Zhong
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  2. Ruochan Chen
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Editors and Affiliations

  1. Centre de Recherche des Cordeliers, Université de Paris, Paris, France

    Guido Kroemer

  2. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Daolin Tang

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Zhong, X., Chen, R. (2023). Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3433-2_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3432-5

  • Online ISBN: 978-1-0716-3433-2

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