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Isolation and Identification of Bifidobacterium spp.

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Postbiotics

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

Taxonomically classified as members of the Bifidobacteriaceae family, bifidobacteria are symbiotic microorganisms that inhabit the intestinal tract of warm-blooded mammals. The members of bifidobacterial species have been isolated from various diverse ecological niches, such as mothers’ milk, infants’ or adults’ stools, human blood, oral cavity, sewage, fermented or raw milk, and hindgut of birds and social insects. This chapter aims to present a comprehensive protocol for culture-dependent enumeration, isolation, and biochemical and molecular identification of Bifidobacterium spp.

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References

  1. Poupard JA, Husain I, Norris RF (1973) Biology of the bifidobacteria. Bacteriol Rev 37:136e165

    Article  Google Scholar 

  2. Orla-Jensen MLD (1924) La classification des bactéries lactiques. Lait 4:468e474

    Google Scholar 

  3. Ventura M, Canchaya C, Tauch A, Chandra G, Fitzgerald GF, Chater KF et al (2007) Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. Microbiol Mol Biol Rev 71(3):495–548

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Scardovi V (1986) Bifidobacterium. In: Sneath PH, Mair NS, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 2, 9th edn. Williams and Wilkins Publishers, Baltimore

    Google Scholar 

  5. Roy D (2001) Media for the isolation and enumeration of bifidobacteria in dairy products. Int J Food Microbiol 69:167–182

    Article  CAS  PubMed  Google Scholar 

  6. Alessandri G, Sinderen D, Ventura M (2021) The genus Bifidobacterium: from genomics to functionality of an important component of the mammalian gut microbiota. Comput Struct Biotechnol J 19:1472–1487

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Roy D (2003) Media for the detection and enumeration of bifidobacteria in food products. In: Corry JEL et al (eds) Handbook of culture media for food microbiology. Elsevier

    Google Scholar 

  8. Hartemink R, Kok BJ, Weenk GH, Rombouts EM (1996) Raffinose-Bifidobacterium (RB) agar, a new selective medium for bifidobacteria. J Microbiol Methods 27:33–43

    Article  CAS  Google Scholar 

  9. Miller TL, Wolin MJ (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27:985e987

    Article  Google Scholar 

  10. Jena R, Choudhury PK, Puniya AK, Tomar SD (2017) Isolation and species delineation of genus Bifidobacterium using PCR-RFL of partial hsp60 gene fragment. LWT- Food Sci Technol 80:286–293

    Article  CAS  Google Scholar 

  11. Coico R (2005) Gram staining. Curr Protoc Microbiol. https://doi.org/10.1002/9780471729259.mca03cs00

  12. Sànchez B, Noriega L, Ruas-Madiedo P, de los Reyes-Gavilàn C, Margolles A (2004) Acquired resistance to bile increases fructose-6-phosphate phosphoketolase activity in Bifidobacterium. FEMS Microbiol Lett 235:35–34

    Article  PubMed  Google Scholar 

  13. Orban JI, Patterson JA (2000) Modification of the phosphoketolase assay for rapid identification of bifidobacterial. J Microbiol Methods 40:221–224

    Article  CAS  PubMed  Google Scholar 

  14. Hadadji M, Benam R, Saidi N, Hanni DE, Kihal M (2005) Identification of cultivable Bifidobacterium species isolated from breastfed infants feces in West-Algeria. Afr J Biotechnol 4(5):422–430

    CAS  Google Scholar 

  15. Miteva V, Abadjieva A, Grigorova R (1991) Differentiation among strains and serotypes of Bacillus thuringiensis by M13 DNA fingerprinting. J Gen Microbiol 137:593–600

    Article  CAS  Google Scholar 

  16. Cancilla MR, Powell IB, Hillier AJ, Davidson BE (1992) Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labels. Appl Environ Microbiol:1772–1775

    Google Scholar 

  17. Raeisi SN, Ouoba LII, Farahmand N, Sutherland J, Hamid B, Ghoddusi HB (2013) Variation, viability and validity of bifidobacteria in fermented milk products. Food Cont 34:691–697

    Article  CAS  Google Scholar 

  18. Kaufmann P, Pfefferkorn A, Teuber M, Meile L (1997) Identification and quantification of Bifidobacterium species isolated from food with genus specific 16S rRNA-targeted probes by colony hybridisation and PCR. Appl Environ Microbiol 63:1268–1273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Requena T, Burton J, Matsuki T, Munro K, Simon MA, Tanaka R (2002) Identification, detection, and enumeration of human Bifidobacterium species by PCR targeting the transaldolase gene. Appl Environl Microbiol 68:2420e2427

    Google Scholar 

  20. Freitas AC, Hill JE (2017) Quantification, isolation and characterization of Bifidobacterium from the vaginal microbiomes of reproductive aged women. Anaerobe 47:145–156

    Article  PubMed  Google Scholar 

  21. Baffoni L, Stenico V, Strahsburger E, Gaggìa F, Di Gioia D, Modesto M (2013) Identification of species belonging to the Bifidobacterium genus by PCRRFLP analysis of a hsp60 gene fragment. BMC Microbiol 13:149. http://www.biomedcentral.com/1471-2180/13/149

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Matsuki T, Watanabe K, Tanaka R, Fukuda M, Oyaizu H (1999) Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA: gene-targeted species-specific primers. Appl Environ Microbiol 65:4506–4512

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Mullié C, Marie-Francoise Odou M, Singer E, Romond M, Izard D (2003) Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin. FEMS Microbiol Lett 222:129–136

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Çanakkale Onsekiz Mart University, Bayramiç Vocational School, Laboratory Technology Program, Bayramiç, Çanakkale, Turkey

    Başar Uymaz Tezel

  2. Ankara University, Engineering Faculty, Department of Food Engineering, Gölbaşı, Ankara, Turkey

    Pınar Şanlıbaba

Authors
  1. Başar Uymaz Tezel
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  2. Pınar Şanlıbaba
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Correspondence to Başar Uymaz Tezel .

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Editors and Affiliations

  1. Department of Microbiology School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Uymaz Tezel, B., Şanlıbaba, P. (2024). Isolation and Identification of Bifidobacterium spp.. In: Dharumadurai, D. (eds) Postbiotics. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3421-9_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3421-9_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3420-2

  • Online ISBN: 978-1-0716-3421-9

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