Abstract
Glaucoma is an irreversible blinding disease characterized by the loss of retinal ganglion cells. Development of therapeutics relies on suitable models and, given its complex nature, these are typically in vivo models. A widely used model, owing to its relative simplicity, is the microbead model. This model involves the injections of beads into the anterior chamber, which are suitably sized to block the aqueous outflow pathway, leading to an elevation in intraocular pressure and ultimately, retinal ganglion cell death. In this chapter, we describe in detail the materials and methods for modelling glaucoma using microbeads (both magnetic and nonmagnetic).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Levkovitch-Verbin H, Quigley HA, Martin KR et al (2002) Translimbal laser photocoagulation to the trabecular meshwork as a model of glaucoma in rats. Invest Ophthalmol Vis Sci 43:402–410
Morrison JC, Johnson EC, Cepurna WO (2018) Hypertonic saline injection model of experimental glaucoma in rats. Methods Mol Biol 1695:11–21
Shepard AR, Millar JC, Pang I-H et al (2010) Adenoviral gene transfer of active human transforming growth factor-β2 elevates intraocular pressure and reduces outflow Facility in Rodent Eyes. Invest Ophthalmol Vis Sci 51:2067–2076
Pang IH, Millar JC, Clark AF (2015) Elevation of intraocular pressure in rodents using viral vectors targeting the trabecular meshwork. Exp Eye Res 141:33–41
Mead B, Amaral J, Tomarev S (2018) Mesenchymal stem cell–derived small extracellular vesicles promote neuroprotection in rodent models of glaucoma. Invest Ophthalmol Vis Sci 59:702–714
Morgan JE, Tribble JR (2015) Microbead models in glaucoma. Exp Eye Res 141:9–14
Sappington RM, Carlson BJ, Crish SD et al (2010) The microbead occlusion model: a paradigm for induced ocular hypertension in rats and mice. Invest Ophthalmol Vis Sci 51:207–216
Ito YA, Belforte N, Cueva Vargas JL et al (2016) A magnetic microbead occlusion model to induce ocular hypertension-dependent glaucoma in mice. JoVE 109:e53731
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Mead, B. (2023). Microbead-Induced Ocular Hypertension in a Rodent Model of Glaucoma. In: Mead, B. (eds) Retinal Ganglion Cells. Methods in Molecular Biology, vol 2708. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3409-7_8
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3409-7_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3408-0
Online ISBN: 978-1-0716-3409-7
eBook Packages: Springer Protocols