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Phage Display

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Michael Hust

    1. Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Braunschweig, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Theam Soon Lim

    1. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2702)

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About this book

This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

 

Keywords

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Antibody Phage Display

      • Alia Nur, Maren Schubert, Jing Yi Lai, Michael Hust, Yee Siew Choong, Wan Yus Haniff Wan Isa et al.
      Pages 3-12

  3. Construction of Antibody Phage Display Libraries

    1. Front Matter

      Pages 13-13
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    2. Construction of Human Immune and Naive scFv Phage Display Libraries

      • Maximilian Ruschig, Philip Alexander Heine, Viola Fühner, Kilian Johannes Karl Zilkens, Stephan Steinke, Maren Schubert et al.
      Pages 15-37

    3. Construction of Naïve and Immune Human Fab Phage Display Library

      • Jing Yi Lai, Theam Soon Lim
      Pages 39-58

    4. Construction of Synthetic Antibody Phage Display Libraries

      • Kim Anh Giang, Sachdev S. Sidhu, Johan Nilvebrant
      Pages 59-75

    5. Construction of Chicken and Ostrich Antibody Libraries

      • Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt
      Pages 77-92

    6. Construction of Rabbit Immune Antibody Libraries

      • Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim
      Pages 93-106

    7. Isolation and Characterization of Single-Domain Antibodies from Immune Phage Display Libraries

      • Martin A. Rossotti, Frederic Trempe, Henk van Faassen, Greg Hussack, Mehdi Arbabi-Ghahroudi
      Pages 107-147

    8. Phagekines: Directed Evolution and Characterization of Functional Cytokines Displayed on Phages

      • Gertrudis Rojas, Tania Carmenate, Gisela García-Pérez, Dayana Pérez-Martínez
      Pages 149-189

    9. Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly

      • Ashley K. Grahn, Grace L. Allen, Brian K. Kay
      Pages 191-203

    10. Construction of an Ultra-Large Phage Display Library by Kunkel Mutagenesis and Rolling Circle Amplification

      • Renhua R. Huang, Michael Kierny, Veronica Volgina, Makio Iwashima, Christina Miller, Brian K. Kay
      Pages 205-226

    11. Construction of Semisynthetic Shark vNAR Yeast Surface Display Antibody Libraries

      • Harald Kolmar, Julius Grzeschik, Doreen Könning, Simon Krah, Stefan Zielonka
      Pages 227-243

  4. Selection Strategies for Antibodies

    1. Front Matter

      Pages 245-245
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    2. Antibody Selection via Phage Display in Microtiter Plates

      • Stephan Steinke, Kristian Daniel Ralph Roth, Maximilian Ruschig, Nora Langreder, Saskia Polten, Kai-Thomas Schneider et al.
      Pages 247-260

    3. Antibody Selection in Solution Using Magnetic Beads

      • Philip Alexander Heine, Maximilian Ruschig, Nora Langreder, Esther Veronika Wenzel, Maren Schubert, Federico Bertoglio et al.
      Pages 261-274

    4. Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning

      • Theam Soon Lim, Angela Chiew Wen Ch’ng, Brenda Pei Chui Song, Jing Yi Lai
      Pages 275-290

    5. Magnetic Nanoparticle-Based Semi-automated Panning for High-Throughput Antibody Selection

      • Angela Chiew Wen Ch’ng, Zoltán Konthur, Theam Soon Lim
      Pages 291-313

    6. Antibody Selection on Cells Targeting Membrane Proteins

      • Viktor Glaser, Ümran Karsli-Ünal, Maike Hagedorn, Tom Pieper
      Pages 315-325
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Editors and Affiliations

  • Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  • Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia

    Theam Soon Lim

Bibliographic Information

  • Book Title: Phage Display

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Hust, Theam Soon Lim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3381-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3380-9Published: 08 September 2023

  • Softcover ISBN: 978-1-0716-3383-0Due: 09 October 2023

  • eBook ISBN: 978-1-0716-3381-6Published: 07 September 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 605

  • Number of Illustrations: 14 b/w illustrations, 69 illustrations in colour

  • Topics: Biological Techniques

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