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Flow Cytometry Analysis of IL-1 Receptors and Toll-Like Receptors on Platelets and Platelet-Derived Extracellular Vesicles

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Toll-Like Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2700))

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Abstract

Flow cytometry is largely used for the immunophenotyping and quantification of several cell types or related components including platelets and extracellular vesicles. Platelets and platelet-derived extracellular vesicles (PEVs) are receiving increased interest in inflammatory diseases including sepsis. Thus, in this chapter, we will describe protocols for the flow cytometry analysis of platelets, platelet/neutrophils hetero aggregates, and PEVs mainly focusing on the evaluation of the surface expression of some IL-1 receptor (ILR) and Toll-like receptor (TLR) family members.

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Author information

Authors and Affiliations

  1. Flow Cytometry Resource, Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy

    Achille Anselmo & Daniela Boselli

Authors
  1. Achille Anselmo
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  2. Daniela Boselli
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Corresponding author

Correspondence to Achille Anselmo .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Perugia, Perugia, Italy

    Francesca Fallarino

  2. Department of Medicine and Surgery, University of Perugia, Perugia, Italy

    Marco Gargaro

  3. Department of Medicine and Surgery, University of Perugia, Perugia, Italy

    Giorgia Manni

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Anselmo, A., Boselli, D. (2023). Flow Cytometry Analysis of IL-1 Receptors and Toll-Like Receptors on Platelets and Platelet-Derived Extracellular Vesicles. In: Fallarino, F., Gargaro, M., Manni, G. (eds) Toll-Like Receptors. Methods in Molecular Biology, vol 2700. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3366-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3366-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3365-6

  • Online ISBN: 978-1-0716-3366-3

  • eBook Packages: Springer Protocols

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