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Magnetic Nanoparticles for Protein Separation and Purification

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2699))

Abstract

Proteins are essential for various functions such as brain activity and muscle contraction in humans. Even though food is a source of proteins, the bioavailability of proteins in most foods is usually limited due to matrix interaction with other biomolecules. Thus, it is essential to extract these proteins and provide them as a nutraceutical supplement to maintain protein levels and avoid protein deficiency. Hence, protein purification and extraction from natural sources are highly significant in biomedical applications. Chromatography, crude mechanical disruption, use of extractive chemicals, and electrophoresis are some of the methods applied to isolate specific proteins. Even though these methods possess several advantages, they are unable to extract specific proteins with high purity. A suitable alternative is the use of nanoparticles, which can be beneficial in protein purification and extraction. Notably, magnetic iron and iron-based nanoparticles have been employed in protein extraction processes and can be reused via demagnetization due to their magnetic property, smaller size, morphology, high surface-to-volume ratio, and surface charge-mediated property. This chapter is a summary of various magnetic nanoparticles (MNPs) that can be used for the biomolecular separation of proteins.

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Authors and Affiliations

  1. Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

    Vadanasundari Vedarethinam

  2. CQM – Centro de Química da Madeira, MMRG, Universidade da Madeira, Campus da Penteada, Funchal, Portugal

    Jaison Jeevanandam

  3. Faculty of Health Sciences, University of Ottawa, Ottawa, ON, Canada

    Caleb Acquah

  4. Chemical Engineering Department, University of Tennessee, Chattanooga, TN, USA

    Michael K. Danquah

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  1. Vadanasundari Vedarethinam
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  2. Jaison Jeevanandam
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  3. Caleb Acquah
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  4. Michael K. Danquah
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Corresponding author

Correspondence to Michael K. Danquah .

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Editors and Affiliations

  1. Department of Life and Health Sciences, Dundalk Institute of Technology, Dundalk, Louth, Ireland

    Sinéad T Loughran

  2. National Institute for Bioprocessing Research and Training (NIBRT), Blackrock, Dublin, Ireland

    John Joseph Milne

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Vedarethinam, V., Jeevanandam, J., Acquah, C., Danquah, M.K. (2023). Magnetic Nanoparticles for Protein Separation and Purification. In: Loughran, S.T., Milne, J.J. (eds) Protein Chromatography. Methods in Molecular Biology, vol 2699. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3362-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3362-5_8

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