Abstract
IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA has been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and in living macrophages either using a plate reader or a microscope.
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Compan, V., Pelegrín, P. (2023). Measuring IL-1β Processing by Bioluminescence Sensors: Using a Bioluminescence Resonance Energy Transfer Biosensor. In: Pelegrín, P., Di Virgilio, F. (eds) NLR Proteins. Methods in Molecular Biology, vol 2696. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3350-2_3
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DOI: https://doi.org/10.1007/978-1-0716-3350-2_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3349-6
Online ISBN: 978-1-0716-3350-2
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