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Preparation of Cage-Like Micellar Assemblies of Engineered Hemoproteins

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Protein Cages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2671))

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Abstract

Natural protein assemblies have encouraged scientists to create large supramolecular systems consisting of various protein motifs. In the case of hemoproteins containing heme as a cofactor, several approaches have been reported to form artificial assemblies with various structures such as fibers, sheets, networks, and cages. This chapter describes the design, preparation, and characterization of cage-like micellar assemblies for chemically modified hemoproteins including hydrophilic protein units attached to hydrophobic molecules. Detailed procedures are described for constructing specific systems using cytochrome b562 and hexameric tyrosine-coordinated heme protein as hemoprotein units with heme-azobenzene conjugate and poly-N-isopropylacrylamide as attached molecules.

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Acknowledgments

We acknowledge supports from JSPS KAKENHI JP15H05804, JP15K21707, JP20H00403, and JP20H02755.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Japan

    Koji Oohora & Takashi Hayashi

Authors
  1. Koji Oohora
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  2. Takashi Hayashi
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Corresponding authors

Correspondence to Koji Oohora or Takashi Hayashi .

Editor information

Editors and Affiliations

  1. Tokyo Institute Of Technology, Yokohama, Japan

    Takafumi Ueno

  2. Chemical & Biomedical Enginering, Nanyang Technological University, Singapore, Singapore

    Sierin Lim

  3. Nanyang Technological University, Singapore, Singapore

    Kelin Xia

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Oohora, K., Hayashi, T. (2023). Preparation of Cage-Like Micellar Assemblies of Engineered Hemoproteins. In: Ueno, T., Lim, S., Xia, K. (eds) Protein Cages. Methods in Molecular Biology, vol 2671. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3222-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3222-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3221-5

  • Online ISBN: 978-1-0716-3222-2

  • eBook Packages: Springer Protocols

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