Abstract
The renal fibroblast, and phenotypically related myofibroblast, are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behaviour, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary renal fibroblasts from kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail.
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Acknowledgements
The work encompassed here was supported by a General Grant (Y21G-HEWT) to TH and ES from the Diabetes Australia Research Fund.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Hewitson, T.D., Smith, E.R. (2023). Propagation, Culture, and Characterization of Renal Fibroblasts. In: Hewitson, T.D., Toussaint, N.D., Smith, E.R. (eds) Kidney Research. Methods in Molecular Biology, vol 2664. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3179-9_2
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DOI: https://doi.org/10.1007/978-1-0716-3179-9_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3178-2
Online ISBN: 978-1-0716-3179-9
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