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Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants

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Hemostasis and Thrombosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2663))

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Abstract

Laboratory testing for Factor V Leiden and Prothrombin G20210A genetic variants permits defining the increased relative risk for venous thromboembolism in selected patients. Laboratory DNA testing for these variants may be undertaken by a variety of methods, including fluorescence-based quantitative real-time PCR (qPCR). This is a rapid, simple, robust, and reliable method to identify genotypes of interest. This chapter describes the method that employs PCR amplification of the patient DNA region of interest and genotyping by allele-specific discrimination technology on a quantitative real-time PCR (qPCR) instrument.

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Authors and Affiliations

  1. Clinical Institute of Genomic Medicine, University Medical Centre Ljubljana, Ljubljana, Slovenia

    Tadej Pajič

  2. Faculty of Medicine, University of Maribor, Maribor, Slovenia

    Tadej Pajič

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  1. Tadej Pajič
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Correspondence to Tadej Pajič .

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Editors and Affiliations

  1. School of Medical Sciences, Faculty of Medicine and Health University of Sydney, Westmead Hospital, Westmead, NSW, Australia

    Emmanuel J. Favaloro

  2. Hemostasis & Thrombosis Center, University of California, Davis, Sacramento, CA, USA

    Robert C. Gosselin

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Pajič, T. (2023). Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants. In: Favaloro, E.J., Gosselin, R.C. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 2663. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3175-1_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3175-1_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3174-4

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