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Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocarcinoma

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

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Abstract

Radiation therapy is a cornerstone of cancer treatment worldwide. Unfortunately, in many cases, it does not control tumor growth, and many tumors display treatment resistance. The molecular pathways leading to treatment resistance in cancer have been subject to research for many years. Isogenic cell lines with divergent radiosensitivities are an extremely useful tool to study the molecular mechanisms that underpin radioresistance in cancer research, as they reduce the genetic variation that is present in patient samples and cell lines of different origin, thus allowing the elucidation of molecular determinants of radioresponse. Here, we describe the process of generating an in vitro isogenic model of radioresistant esophageal adenocarcinoma by chronic irradiation of esophageal adenocarcinoma cells with clinically relevant doses of X-ray radiation. We also characterize cell cycle, apoptosis, reactive oxygen species (ROS) production, DNA damage and repair in this model to investigate the underlying molecular mechanisms of radioresistance in esophageal adenocarcinoma.

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Acknowledgments

A.C. and N.L.L. are supported by the Health Research Board, grant numbers ILP-POR-2017-007 and EIA-2017-020.

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Authors and Affiliations

  1. Department of Surgery, Trinity St. James’s Cancer Institute, Trinity Translational Medicine Institute, Dublin, Ireland

    Aoife Cannon, Stephen G. Maher & Niamh Lynam-Lennon

Authors
  1. Aoife Cannon
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  2. Stephen G. Maher
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  3. Niamh Lynam-Lennon
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Corresponding author

Correspondence to Niamh Lynam-Lennon .

Editor information

Editors and Affiliations

  1. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Dania Movia

  2. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Adriele Prina-Mello

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cannon, A., Maher, S.G., Lynam-Lennon, N. (2023). Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocarcinoma. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3055-6

  • Online ISBN: 978-1-0716-3056-3

  • eBook Packages: Springer Protocols

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