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High-Efficiency Single-Cell Electrical Impedance Spectroscopy

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Cell Viability Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2644))

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Abstract

Single-cell impedance measurement is label free and noninvasive in characterizing the electrical properties of single cells. At present, though widely used for impedance measurement, electrical impedance flow cytometry (IFC) and electrical impedance spectroscopy (EIS) are used alone for most microfluidic chips. Here, we describe high-efficiency single-cell electrical impedance spectroscopy, which combines in one chip the IFC and EIS techniques for high-efficiency single-cell electrical property measurement. We envision that the strategy of combining IFC and EIS provides a new thought in the efforts to enhance the efficiency of electrical property measurement for single cells.

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Acknowledgments

This work is supported by the NSFC (no. 62174096, 21727813, and 52105572) and the One Thousand Young Talent Program of China.

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Authors and Affiliations

  1. State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China

    Yongxiang Feng, Peng Zhao, Fei Liang & Wenhui Wang

  2. Anhui Province Key Laboratory of Measuring Theory and Precision Instrument, School of Instrument Science and Optoelectronics Engineering, Hefei University of Technology, Hefei, Anhui, China

    Liang Huang

Authors
  1. Yongxiang Feng
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  2. Liang Huang
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  3. Peng Zhao
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  4. Fei Liang
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  5. Wenhui Wang
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Corresponding author

Correspondence to Wenhui Wang .

Editor information

Editors and Affiliations

  1. Institute of Medical Biotechnology (MBT), Department of Chemical and Biological Engineering (CBI), Friedrich-Alexander-University Erlangen-Nüremberg, Erlangen, Germany

    Oliver Friedrich

  2. Institute of Medical Biotechnology (MBT), Department of Chemical and Biological Engineering (CBI), Friedrich-Alexander-University Erlangen-Nüremberg, Erlangen, Germany

    Daniel F. Gilbert

1 Electronic Supplementary Material

Data 1

DataAcqurie-Labview2018 (VI 310 kb)

Data 2

DataProcessing-matlab-R2016b (M 1 kb)

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Feng, Y., Huang, L., Zhao, P., Liang, F., Wang, W. (2023). High-Efficiency Single-Cell Electrical Impedance Spectroscopy. In: Friedrich, O., Gilbert, D.F. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 2644. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3052-5_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3052-5_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3051-8

  • Online ISBN: 978-1-0716-3052-5

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