Abstract
Gasdermin proteins except DFNB59 are the executioners of pyroptotic cell death. Cleavage of a gasdermin by an active protease causes lytic cell death. Gasdermin C (GSDMC) is cleaved by caspase-8 in response to macrophage-secreted TNF-α. Upon cleavage, the GSDMC-N domain is liberated and oligomerized, followed by pore formation in the plasma membrane. GSDMC cleavage, LDH release, and plasma membrane translocation of GSDMC-N domain are the reliable markers for GSDMC-mediated cancer cell pyroptosis (CCP). Here, we describe the methods for examining GSDMC-mediated CCP.
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Acknowledgments
This work was funded by the Ministry of Science and Technology of Taiwan (MOST 110-2639-B-039-001-ASP to M.-C. H), Cancer Center Support Grant (NCI NIH P30 CA016672 to M.-C. H.), and a grant from the National Natural Science Foundation of China (82173107).
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Hou, J., Wang, S., Miao, R., Zhang, X., Hung, MC. (2023). Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis. In: Fink, S.L. (eds) Pyroptosis. Methods in Molecular Biology, vol 2641. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3040-2_11
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DOI: https://doi.org/10.1007/978-1-0716-3040-2_11
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3040-2
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