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Methods for Characterizing Flavobacterium in Fish

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Aquaculture Microbiology

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Abstract

Bacterial gill disease, rainbow trout fry syndrome, Columnaris disease, and bacterial cold-water disease are caused by bacteria that are pathogenic to fish. They belong to the Flavobacteriaceae family. They are spotted with yellow pigmentation in aquatic organisms. These kinds of bacteria are isolated and identified using techniques such as Gram staining, motility, gliding motility, biochemical tests, and API 20E. It can also be identified using technical methods by plate count and microscopy. Molecular characterization such as DNA extraction, polymerase chain reaction (PCR), and phylogenetic analysis is also done to identify the species.

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Acknowledgments

The work is supported by the Ministry of Earth Sciences grant (MoES) (MoES/36/OOIS/53/2016), Govt. of India, and Sathyabama Institute of Science and Technology.

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Authors and Affiliations

  1. Centre for Ocean Research, Col. Dr. Jeppiaar Ocean Research Field Facility, Sathyabama Institute of Science and Technology (Deemed to be University), Chennai, Tamilnadu, India

    Yamini Gopi, Sakthinarenderan Sai, Mirunalini Ganesan & Ravi Mani

Authors
  1. Yamini Gopi
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  2. Sakthinarenderan Sai
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  3. Mirunalini Ganesan
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  4. Ravi Mani
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Corresponding author

Correspondence to Ravi Mani .

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Editors and Affiliations

  1. Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore, Tamil Nadu, India

    John Thomas

  2. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Natarajan Amaresan

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Gopi, Y., Sai, S., Ganesan, M., Mani, R. (2023). Methods for Characterizing Flavobacterium in Fish. In: Thomas, J., Amaresan, N. (eds) Aquaculture Microbiology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3032-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3032-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3031-0

  • Online ISBN: 978-1-0716-3032-7

  • eBook Packages: Springer Protocols

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