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Generation of Rabbit Chimeras by Eight-Cell Stage Embryo Injection

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Transgenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2631))

Abstract

Pluripotent stem cell (PSC) injection to the blastocyst stage embryos is a widely used method to evaluate the pluripotency through chimeric contribution. It is routinely used to produce transgenic mice. However, PSC injection to the blastocyst stage embryos in rabbits is challenging. At this stage, the in vivo developed rabbit blastocysts possess a thick mucin layer that is inhibitory for microinjection, whereas in vitro developed rabbit blastocysts that lack such mucin layer often fail to implant after embryo transfer. In this chapter, we describe a detailed protocol of rabbit chimera production through mucin-free eight-cell stage embryo injection procedure.

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Author information

Authors and Affiliations

  1. Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI, USA

    Dongshan Yang, Jun Song, Jie Xu, Jifeng Zhang & Y. Eugene Chen

  2. Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, China

    Jun Song

Authors
  1. Dongshan Yang
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  2. Jun Song
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  3. Jie Xu
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  4. Jifeng Zhang
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  5. Y. Eugene Chen
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Corresponding authors

Correspondence to Dongshan Yang or Y. Eugene Chen .

Editor information

Editors and Affiliations

  1. Transgenic Animal Model Core, University of Michigan, Ann Arbor, MI, USA

    Thomas L. Saunders

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yang, D., Song, J., Xu, J., Zhang, J., Chen, Y.E. (2023). Generation of Rabbit Chimeras by Eight-Cell Stage Embryo Injection. In: Saunders, T.L. (eds) Transgenesis. Methods in Molecular Biology, vol 2631. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2990-1_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2990-1_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2989-5

  • Online ISBN: 978-1-0716-2990-1

  • eBook Packages: Springer Protocols

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