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Serum/Plasma Proteomics

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. David W. Greening

    1. Molecular Proteomics, Baker Heart and Diabetes Institute, Melbourne, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Richard J. Simpson

    1. Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe Institute for Molecular Science, Melbourne, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2628)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Blood Processing and Handling Strategies

    1. Front Matter

      Pages 1-1
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    2. Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion

      • Rosemary L. Sparrow, Richard J. Simpson, David W. Greening
      Pages 3-18

    3. Collection of Plasma Samples in Areas with Limited Healthcare Access

      • Alicia Johnson, Camila Braga, Pedro de Magalhães Padilha, Jiri Adamec
      Pages 19-32

    4. Blood Collection Processing and Handling for Plasma and Serum Proteomics

      • Conor McCafferty, Natasha Letunica, Ella Swaney, Cai Tengyi, Paul Monagle, Vera Ignjatovic et al.
      Pages 33-40

    5. Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis

      • Rosemary L. Sparrow, Richard J. Simpson, David W. Greening
      Pages 41-49

  3. Discovery-Based Mass Spectrometry

    1. Front Matter

      Pages 51-51
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    2. High-Throughput Proteome Profiling of Plasma and Native Plasma Complexes Using Native Chromatography

      • Aleksandr Gaun, Niclas Olsson, John C. K. Wang, Dan L. Eaton, Fiona E. McAllister
      Pages 53-79

    3. High-Throughput and In-Depth Proteomic Profiling of 5 μL Plasma and Serum Using TMTpro 16-Plex

      • Yan Zhou, Rui Sun, Sainan Li, Xiao Liang, Liujia Qian, Liang Yue et al.
      Pages 81-92

    4. An Optimized Data-Independent Acquisition Strategy for Comprehensive Analysis of Human Plasma Proteome

      • Haoyun Fang, David W. Greening
      Pages 93-107

    5. In-Depth Blood Proteome Profiling by Extensive Fractionation and Multiplexed Quantitative Mass Spectrometry

      • Xue Zhang, Huan Sun, Zhen Wang, Suiping Zhou, Yingxue Fu, High A. Anthony et al.
      Pages 109-125

    6. Early Cancer Biomarker Discovery Using DIA-MS Proteomic Analysis of EVs from Peripheral Blood

      • Camila Espejo, Bruce Lyons, Gregory M. Woods, Richard Wilson
      Pages 127-152

  4. Developments in Technologies for Plasma Proteomics

    1. Front Matter

      Pages 153-153
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    2. Strategies to Enrich, Identify, and Characterize Glycoproteome in Blood Plasma Using Liquid Chromatography High-Resolution Mass Spectrometry

      • Saravanan Kumar
      Pages 155-172

    3. Proteome Analysis of Whole Blood Collected by Volumetric Absorptive Microsampling

      • Mark P. Molloy, Cameron Hill, Matthew J. McKay, Ben R. Herbert
      Pages 173-179

    4. Proteomic Applications and Considerations: From Research to Patient Care

      • Natasha Letunica, Conor McCafferty, Ella Swaney, Tengyi Cai, Paul Monagle, Vera Ignjatovic et al.
      Pages 181-192

  5. Enrichment & Detection Strategies

    1. Front Matter

      Pages 193-193
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    2. Immunoaffinity Mass Spectrometry Diagnostic Tests for Multi-Biomarker Assays

      • Scott Bringans, Tammy Casey, Jun Ito, Tasha Lumbantobing, Ronan O’Neill, Richard Lipscombe
      Pages 195-206

    3. Secretome Profile of Leukocyte-Platelet-Rich Fibrin (L-PRF) Membranes

      • Lidia Hermida-Nogueira, Juan Blanco, Ángel García
      Pages 207-219

    4. Quantification of Proteins in Blood by Absorptive Microtiter Plate-Based Affinity Purification Coupled to Liquid Chromatography-Mass Spectrometry

      • Frank Klont, Oladapo Olaleye, Rainer Bischoff
      Pages 221-233
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Keywords

About this book

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma.

 

Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

 

Editors and Affiliations

  • Molecular Proteomics, Baker Heart and Diabetes Institute, Melbourne, Australia

    David W. Greening

  • Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe Institute for Molecular Science, Melbourne, Australia

    Richard J. Simpson

About the editors

- 

Bibliographic Information

  • Book Title: Serum/Plasma Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: David W. Greening, Richard J. Simpson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2978-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2977-2Published: 14 February 2023

  • Softcover ISBN: 978-1-0716-2980-2Published: 14 February 2024

  • eBook ISBN: 978-1-0716-2978-9Published: 13 February 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVIII, 596

  • Number of Illustrations: 10 b/w illustrations, 50 illustrations in colour

  • Topics: Chemistry/Food Science, general, Bioinformatics, Mass Spectrometry

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