Abstract
Antigen cross-presentation by dendritic cells is an important pathway to prime CD8+ T cells in infections, cancer, and other immune-mediated pathologies. Particularly in cancer, cross-presentation of tumor-associated antigens is crucial for an effective antitumor CTL response. The mostly accepted cross-presentation assay is to use chicken ovalbumin (OVA) as a model antigen and then utilize OVA-specific TCR transgenic CD8+ T (OT-I) cells to measure the cross-presenting capacity. Here we describe in vivo and in vitro assays to measure the function of antigen cross-presentation using cell-associated OVA.
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Acknowledgements
This work is supported by the National Key R&D Program of China (2018YFA0508300) and the National Natural Science Foundation of China (31570863) to C.Y.Y.
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Ou, P., Wen, L., Ni, H., Yang, C.Y. (2023). In Vivo and In Vitro Assay to Address Dendritic Cell Antigen Cross-Presenting Capacity. In: Sisirak, V. (eds) Dendritic Cells. Methods in Molecular Biology, vol 2618. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2938-3_17
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DOI: https://doi.org/10.1007/978-1-0716-2938-3_17
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