Abstract
Mitochondria are eukaryotic organelles of endosymbiotic origin that contain their own genetic material, mitochondrial DNA (mtDNA), and dedicated systems for mtDNA maintenance and expression. MtDNA molecules encode a limited number of proteins that are nevertheless all essential subunits of the mitochondrial oxidative phosphorylation system. Here, we describe protocols to monitor DNA and RNA synthesis in intact, isolated mitochondria. These in organello synthesis protocols are valuable techniques for studying the mechanisms and regulation of mtDNA maintenance and expression.
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Misic, J., Milenkovic, D. (2023). Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria. In: Nicholls, T.J., Uhler, J.P., Falkenberg, M. (eds) Mitochondrial DNA. Methods in Molecular Biology, vol 2615. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2922-2_16
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DOI: https://doi.org/10.1007/978-1-0716-2922-2_16
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2921-5
Online ISBN: 978-1-0716-2922-2
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