Abstract
Our increased understanding of how a cell’s microenvironment influences its behavior has fueled an interest in three-dimensional (3D) cell cultures for drug discovery. Particularly, scaffold-based 3D cultures are expected to recapitulate in vivo tissue stiffness and extracellular matrix composition more accurately than standard two-dimensional (2D) monolayer cultures. Here we present a 3D hydrogel cell culture setup suitable for automated screening with standard high-throughput screening (HTS) liquid handling equipment commonly found in a drug discovery laboratory. Further, we describe the steps required to validate the assay system for compound screening.
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Acknowledgments
We thank Katherine Drake for critical reading of the manuscript. This work was supported by the DoBelieve Foundation, the Nemours Foundation, National Institutes of Health grant 1R01CA263216-01A1, and an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number U54-GM104941 (PI: Hicks).
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Sperle, K., Pochan, D.J., Langhans, S.A. (2023). 3D Hydrogel Cultures for High-Throughput Drug Discovery. In: Ursini-Siegel, J. (eds) The Tumor Microenvironment. Methods in Molecular Biology, vol 2614. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2914-7_22
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DOI: https://doi.org/10.1007/978-1-0716-2914-7_22
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