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Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes

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Glycolipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2613))

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Abstract

Gangliosides play a variety of physiological roles and are one of the most important lipid raft constituents. However, their dynamic behaviors have scarcely been investigated in living cells because of the lack of fluorescent probes that behave like their parental molecules. Recently, fluorescent ganglioside probes that mimic native ganglioside behaviors have been developed. In this chapter, I discuss the recent advances in research related to the lateral localization and dynamic behaviors of gangliosides in the plasma membranes of living cells.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Specific Research Kiban B (21H02424) and for Innovative Areas (20 K21387) from the Japan Society for the Promotion of Science, by a grant from Core Research for Evolutional Science and Technology (CREST project of “Elucidation of Biological Mechanism of Extracellular Fine Particles and the Control System”) of the Japan Science and Technology Agency (JPMJCR18H2), and by a grant from the Takeda Foundation. WPI-iCeMS of Kyoto University is supported by the World Premiere Research Center Initiative (WPI) of the ΜEXT.

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Authors and Affiliations

  1. Institute for Glyco-core Research (iGCORE), Gifu University, Gifu, Japan

    Kenichi G. N. Suzuki

  2. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan

    Kenichi G. N. Suzuki

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  1. Kenichi G. N. Suzuki
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Correspondence to Kenichi G. N. Suzuki .

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Editors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan

    Kazuya Kabayama

  2. Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    Jin-ichi Inokuchi

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Suzuki, K.G.N. (2023). Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes. In: Kabayama, K., Inokuchi, Ji. (eds) Glycolipids. Methods in Molecular Biology, vol 2613. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2910-9_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2910-9_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2909-3

  • Online ISBN: 978-1-0716-2910-9

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