Abstract
Carbohydrates have a great potential in generating structural and immunological diversities. Microbial pathogens often decorate their outmost surfaces with specific carbohydrate signatures. Carbohydrate antigens also differ significantly from protein antigens in physiochemical properties, especially in surface display of antigenic determinants in aqueous solutions. Technical optimization or modifications are often needed when we apply standard procedures for protein-based enzyme-linked immunosorbent assay (ELISA) to assess immunologically potent carbohydrates. We present here our laboratory protocols for performing carbohydrate ELISA and discuss several assay platforms that may be applied complementarily to explore the carbohydrate moieties that are critical for host immune recognition and induction of glycan-specific antibody responses.
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Acknowledgments
We acknowledge the Kabat Collection of Carbohydrate Antigens at SRI International for a panel of carbohydrate antigens and antibodies that were applied in this study. This work was supported in part by the US Government grants R21DA046144-02 (NIH), 1R21CA254048-01 (NIH), and PR170128 (CDMRP) to DW. The content is solely the responsibility of the author and does not necessarily represent the official views of the funding agents.
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Wang, D. (2023). Assessment of Immunologically Potent Carbohydrates. In: Matson, R.S. (eds) ELISA. Methods in Molecular Biology, vol 2612. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2903-1_10
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DOI: https://doi.org/10.1007/978-1-0716-2903-1_10
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