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High-Throughput Screening of Fosmid Libraries for Increased Identification of Novel N-Acyl Homoserine Lactone Degrading Enzymes

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Microbial Environmental Genomics (MEG)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2605))

Abstract

Functional metagenomics is an essential and effective approach to recover new enzymes from the environment. In this chapter, we describe a procedure to construct metagenomic library to discover new N-acyl homoserine lactone (AHL) degrading enzymes based on a direct method or an indirect enrichment procedure. Applicable to any bacterial ecosystem, it enables rapid identification of functional enzymes effective to degrade AHLs.

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Acknowledgements

This research was funded by the INRA metaprogramme (project Metascreen) and the Labex ARBRE (ANR-11-LABX-0002-01).

Author information

Authors and Affiliations

  1. Université de Lorraine, INRAE, UMR1136 “Interactions Arbres-Microorganismes”, F-54280 Champenoux, France

    Stephane Uroz

  2. INRAE, UR1138 “Biogéochimie des écosystèmes forestiers”, F-54280 Champenoux, France

    Stephane Uroz

  3. INRAE, USC1371 “Labex ARBRE”, F-54280 Champenoux, France

    Stephane Uroz

  4. Université de Lyon, INSA Lyon, CNRS, UMR5240 Microbiologie Adaptation et Pathogénie, Villeurbanne, France

    Philippe Oger

Authors
  1. Stephane Uroz
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  2. Philippe Oger
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Corresponding author

Correspondence to Stephane Uroz .

Editor information

Editors and Affiliations

  1. Tree-Microbe Interact (IAM); Biogeochem, UMR1136 INRA/University of Lorraine, Labex ARB, Champenoux, France

    Francis Martin

  2. Université de Lorraine, INRAE, UMR1136 “Interactions Arbres-Microorganismes”, F-54280 Champenoux, France

    Stephane Uroz

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Uroz, S., Oger, P. (2023). High-Throughput Screening of Fosmid Libraries for Increased Identification of Novel N-Acyl Homoserine Lactone Degrading Enzymes. In: Martin, F., Uroz, S. (eds) Microbial Environmental Genomics (MEG). Methods in Molecular Biology, vol 2605. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2871-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2871-3_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2870-6

  • Online ISBN: 978-1-0716-2871-3

  • eBook Packages: Springer Protocols

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