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Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens

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The Plant Cytoskeleton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2604))

Abstract

Protonemata of the moss Physcomitrium patens are ideal structures in which to observe cytoskeletal organization and dynamics. Special care is needed to prepare P. patens cultures for high-resolution microscopy. Here, we describe methods for spinning disk microscopy of dividing P. patens cells expressing sGFP-tubulin and H2B-mCherry, including detailed methods for culturing P. patens.

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Acknowledgments

We would like to thank Takumi Kataoka (Miyagi University) for his technical assistance. This research was supported by JSPS KAKENHI (18H02471 to TM, 19K06726 to YH).

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Authors and Affiliations

  1. Department of Applied Bioscience, Kanagawa Institute of Technology, Atsugi, Japan

    Takashi Murata

  2. School of Food Industrial Sciences, Miyagi University, Sendai, Japan

    Yuji Hiwatashi

Authors
  1. Yuji Hiwatashi
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  2. Takashi Murata
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Corresponding author

Correspondence to Takashi Murata .

Editor information

Editors and Affiliations

  1. Department of Biosciences, University of Durham, Durham, UK

    Patrick J. Hussey

  2. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    Pengwei Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Hiwatashi, Y., Murata, T. (2023). Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens. In: Hussey, P.J., Wang, P. (eds) The Plant Cytoskeleton. Methods in Molecular Biology, vol 2604. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2867-6_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2867-6_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2866-9

  • Online ISBN: 978-1-0716-2867-6

  • eBook Packages: Springer Protocols

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