Abstract
Expansion microscopy enables super-resolved visualization of specimen without the need of highly sophisticated and expensive optical instruments. Instead, the method is executed with conventional chemicals and lab equipment. ImagingĀ of bacteria is performed using standard fluorescence microscopy. This chapter describes a protocol for the expansion microscopy of Bacillus subtilis expressing DivIVA-GFP. In addition, the cell wall was labeled by wheat germ agglutinin. Here, we place emphasis on the challenges of selecting the protein and organism of interest.
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Acknowledgments
The authors appreciate funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation): Project-ID 398967434 -TRR261 (TRR 261 project Z02).
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Middelhauve, V., Siebrasse, J.P., Kubitscheck, U. (2023). Expansion Microscopy of Bacillus subtilis. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 2601. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2855-3_10
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DOI: https://doi.org/10.1007/978-1-0716-2855-3_10
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