Abstract
Cancer cells possess a remarkable capacity to dissociate from a primary tumor, invade the surrounding tissues and vasculature, and eventually form metastases in distant organs. This complex and multistep process remains one of the major causes of mortality in cancer patients worldwide. Multiple studies have highlighted the role of actin-rich structures called invadopodia (“invasive feet”), which adhere to the matrix, contain and secrete matrix-degrading proteinases, and apply protrusive forces generated by the actin cytoskeleton, which drive the invasive process. Here, we describe a fluorescent microscopy-based protocol for imaging and quantifying both invadopodia formation and matrix degradation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dillekas H, Rogers MS, Straume O (2019) Are 90% of deaths from cancer caused by metastases? Cancer Med 8(12):5574–5576
Riggi N, Aguet M, Stamenkovic I (2018) Cancer metastasis: a reappraisal of its underlying mechanisms and their relevance to treatment. Annu Rev Pathol 13:117–140
Lambert AW, Pattabiraman DR, Weinberg RA (2017) Emerging biological principles of metastasis. Cell 168(4):670–691
Pachmayr E, Treese C, Stein U (2017) Underlying mechanisms for distant metastasis - molecular biology. Visceral medicine 33(1):11–20
Eccles SA, Welch DR (2007) Metastasis: recent discoveries and novel treatment strategies. Lancet 369(9574):1742–1757
Sun Y, Ma L (2015) The emerging molecular machinery and therapeutic targets of metastasis. Trends Pharmacol Sci 36(6):349–359
Welch DR, Hurst DR (2019) Defining the hallmarks of metastasis. Cancer Res 79(12):3011–3027
Revach OY, Grosheva I, Geiger B (2020) Biomechanical regulation of focal adhesion and invadopodia formation. J Cell Sci 133(20)
Paz H, Pathak N, Yang J (2014) Invading one step at a time: the role of invadopodia in tumor metastasis. Oncogene 33(33):4193–4202
Ayala I, Baldassarre M, Caldieri G, Buccione R (2006) Invadopodia: a guided tour. Eur J Cell Biol 85(3–4):159–164
Murphy DA, Courtneidge SA (2011) The ‘ins’ and ‘outs’ of podosomes and invadopodia: characteristics, formation and function. Nat Rev Mol Cell Biol 12(7):413–426
Chen WT, Olden K, Bernard BA, Chu FF (1984) Expression of transformation-associated protease(s) that degrade fibronectin at cell contact sites. J Cell Biol 98(4):1546–1555
Acknowledgments
The studies described herein are funded by the Israel Science Foundation. The authors are grateful to Dr. Or-Yam Revach for her contribution to the development of the assay in our laboratory.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Venghateri, J.B., Geiger, B. (2023). Quantification of Invadopodia Formation and Matrix Degradation Activity. In: Zaidel-Bar, R. (eds) Mechanobiology. Methods in Molecular Biology, vol 2600. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2851-5_12
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2851-5_12
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2850-8
Online ISBN: 978-1-0716-2851-5
eBook Packages: Springer Protocols