Abstract
The specificity in gene regulation is controlled by interactions between transcription factors (TFs) and genomic DNA regions such as promoters and enhancers. Enhanced yeast one-hybrid (eY1H) assays are among the methods used for high-throughput detection of transcription factor–DNA interactions. Here, we describe the procedure for screening interactions between DNA regions of interest (“DNA-baits”) and an array of transcription factors (“TF-preys”), after DNA-bait and TF-prey yeast strains have been generated. Using a high-density array robotic platform, this method can be used to screen interactions between multiple DNA regions and >1000 TFs within a single experiment.
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Acknowledgments
This work was supported by NIH grant R35 GM128625 to J.I.F.B..
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Berenson, A., Fuxman Bass, J.I. (2023). Enhanced Yeast One-Hybrid Assays to Study Protein–DNA Interactions. In: Simoes-Costa, M. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 2599. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2847-8_2
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DOI: https://doi.org/10.1007/978-1-0716-2847-8_2
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