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Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2599))

Abstract

Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions. Preparation of reconstituted nucleosomes allows for the study of nucleosomes with defined compositions. Here, we describe methods for refolding recombinant human histones, reconstituting nucleosome core particles with 147 bp Widom 601 DNA, and purification via sucrose gradient.

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Acknowledgments

Research reported in this publication was supported in part by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM142594 and the Medical College of Wisconsin Research Affairs Committee. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA

    Erin F. Hammonds & Emma A. Morrison

Authors
  1. Erin F. Hammonds
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  2. Emma A. Morrison
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Corresponding author

Correspondence to Emma A. Morrison .

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Editors and Affiliations

  1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA

    Marcos Simoes-Costa

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Hammonds, E.F., Morrison, E.A. (2023). Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. In: Simoes-Costa, M. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 2599. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2847-8_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2847-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2846-1

  • Online ISBN: 978-1-0716-2847-8

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