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The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

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Cartilage Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2598))

Abstract

The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

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Author information

Authors and Affiliations

  1. AO Research Institute Davos, Davos Platz, Switzerland

    Yann D. Ladner & Mauro Alini

  2. Institute for Biomechanics, ETH Zurich, Zurich, Switzerland

    Yann D. Ladner

  3. UCB Pharma, Slough, UK

    Angela R. Armiento

Authors
  1. Yann D. Ladner
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  2. Mauro Alini
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  3. Angela R. Armiento
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Corresponding author

Correspondence to Mauro Alini .

Editor information

Editors and Affiliations

  1. AO Research Institute Davos, Davos Platz, Switzerland

    Martin J. Stoddart

  2. AO Research Institute Davos, Davos Platz, Switzerland

    Elena Della Bella

  3. UCB Pharma, Slough, UK

    Angela R. Armiento

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ladner, Y.D., Alini, M., Armiento, A.R. (2023). The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans. In: Stoddart, M.J., Della Bella, E., Armiento, A.R. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 2598. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2839-3_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2839-3_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2838-6

  • Online ISBN: 978-1-0716-2839-3

  • eBook Packages: Springer Protocols

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