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Detection of MicroRNAs in Brain Slices Using In Situ Hybridization

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MicroRNA Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2595))

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Abstract

MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles have been demonstrated for these noncoding RNAs in various neurological disorders. To this end, visualization of microRNAs in specific cell types and subcellular compartments within tissue sections provides researchers with essential insights that support understanding of the cell and molecular mechanisms of microRNAs in brain diseases. In this chapter we describe an in situ hybridization protocol for the detection of microRNAs in mouse brain sections, which provides cellular resolution of the expression of microRNAs in the brain.

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References

  1. Guo H, Ingolia NT, Weissman JS et al (2010) Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 466:835–840

    Article  CAS  Google Scholar 

  2. Schratt G (2009) microRNAs at the synapse. Nat Rev Neurosci 10:842–849

    Article  CAS  Google Scholar 

  3. McNeill E, Van Vactor D (2012) MicroRNAs shape the neuronal landscape. Neuron 75:363–379

    Article  CAS  Google Scholar 

  4. Bartel DP (2004) MicroRNAs: genomics, bio- genesis, mechanism, and function. Cell 116:281–297

    Article  CAS  Google Scholar 

  5. Wang Y, Medvid R, Melton C et al (2007) DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self- renewal. Nat Genet 39:380–385

    Article  CAS  Google Scholar 

  6. Tang F, Kaneda M, O’Carroll D et al (2007) Maternal microRNAs are essential for mouse zygotic development. Genes Dev 21:644–648

    Article  CAS  Google Scholar 

  7. Giraldez AJ, Cinalli RM, Glasner ME et al (2005) MicroRNAs regulate brain morpho- genesis in zebrafish. Science 308:833–838

    Article  CAS  Google Scholar 

  8. Kawase-Koga Y, Otaegi G, Sun T (2009) Different timings of Dicer deletion affect neu- rogenesis and gliogenesis in the developing mouse central nervous system. Dev Dyn 238:2800–2812

    Article  Google Scholar 

  9. Sun E, Shi Y (2015) MicroRNAs: small molecules with big roles in neurodevelopment and diseases. Exp Neurol 268:46–53

    Article  CAS  Google Scholar 

  10. Nowak JS, Michlewski G (2013) miRNAs in development and pathogenesis of the nervous system. Biochem Soc Trans 41:815–820

    Article  CAS  Google Scholar 

  11. Jimenez-Mateos EM, Engel T, Merino- Serrais P et al (2012) Silencing microRNA-134 produces neuroprotective and prolonged seizure-suppressive effects. Nat Med 18:1087–1094

    Article  CAS  Google Scholar 

  12. Bicker S, Khudayberdiev S, Weiss K et al (2013) The DEAH-box helicase DHX36 mediates dentritic localization of the neuronal precursor-microRNA-134. Genes Dev 27:991–996

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland

    Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall & Eva M. Jimenez-Mateos

Authors
  1. Sean Quinlan
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  2. Christine Henke
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  3. Gary P. Brennan
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  4. David C. Henshall
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  5. Eva M. Jimenez-Mateos
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Corresponding author

Correspondence to Eva M. Jimenez-Mateos .

Editor information

Editors and Affiliations

  1. Department of Science, South East Technological University, Waterford, Ireland

    Sweta Rani

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Quinlan, S., Henke, C., Brennan, G.P., Henshall, D.C., Jimenez-Mateos, E.M. (2023). Detection of MicroRNAs in Brain Slices Using In Situ Hybridization. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 2595. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2823-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2823-2_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2822-5

  • Online ISBN: 978-1-0716-2823-2

  • eBook Packages: Springer Protocols

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