Abstract
Novel optical tissue clearing techniques enable three-dimensional imaging of entire organs at a subcellular resolution while preserving tissue architecture and fluorescence. In conjunction with computational image segmentation and automated analysis, these techniques provide fast and precise three-dimensional morphometry. Here, we present a tissue clearing protocol adapted to nerves and their motor and sensory targets in experimental rat models. Given their rapid processing times, low costs, and wide-ranging applicability, these techniques are likely to be a key technology for future nerve repair studies.
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Acknowledgments
This work was supported by the German Research Foundation (DA 2255/1-1).
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Daeschler, S.C., Borschel, G.H. (2023). Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research. In: Kalyuzhny, A.E. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 2593. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2811-9_10
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DOI: https://doi.org/10.1007/978-1-0716-2811-9_10
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