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Investigating Physiopathological Roles for Sirtuins in a Mouse Model

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HDAC/HAT Function Assessment and Inhibitor Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2589))

Abstract

Sirtuins are identified as NAD+-dependent class III histone deacetylases (HDAC) and are involved in a variety of cellular activities, including energy metabolism, DNA repair, epigenetics, gene expression, cell proliferation, differentiation, and survival. Using genetically modified model organisms, sirtuins are proved to be one of the most conserved aging-regulatory and longevity-promoting genes/pathways among species. Of the seven sirtuins, SIRT7 is the only sirtuin that localizes in the nucleolus. SIRT7 senses endogenous and environmental stress to maintain physiological homeostasis. Sirt7 deficient and transgenic mice provide a useful tool to understand the mechanisms of aging and related pathologies. In this chapter, we summarized the most widely applied methods to understand the physiopathological function of SIRT7 in mice.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (grant no. 82061160495 to BL) and the Shenzhen Municipal Commission of Science and Technology Innovation (grant no. ZDSYS20190902093401689).

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Authors and Affiliations

  1. Shenzhen Key Laboratory of Systemic Aging and Intervention (SKL-SAI), School of Basic Medical Sciences, Shenzhen University, Shenzhen, China

    Shimin Sun, Xiaojiao Xia, Ming Wang & Baohua Liu

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  1. Shimin Sun
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  2. Xiaojiao Xia
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  3. Ming Wang
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  4. Baohua Liu
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Correspondence to Baohua Liu .

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Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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Sun, S., Xia, X., Wang, M., Liu, B. (2023). Investigating Physiopathological Roles for Sirtuins in a Mouse Model. In: Krämer, O.H. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 2589. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2788-4_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2788-4_7

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