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Radiometric Assay of NAPE-PLD Activity

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2576))

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Abstract

N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).

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References

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Author information

Authors and Affiliations

  1. Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy

    Filomena Fezza & Nicolina Mastrangelo

  2. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Mauro Maccarrone

  3. European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy

    Mauro Maccarrone

Authors
  1. Filomena Fezza
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  2. Nicolina Mastrangelo
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  3. Mauro Maccarrone
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Corresponding author

Correspondence to Filomena Fezza .

Editor information

Editors and Affiliations

  1. Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy

    Mauro Maccarrone

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fezza, F., Mastrangelo, N., Maccarrone, M. (2023). Radiometric Assay of NAPE-PLD Activity. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2728-0_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2727-3

  • Online ISBN: 978-1-0716-2728-0

  • eBook Packages: Springer Protocols

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