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Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting

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Brain Organoid Research

Part of the book series: Neuromethods ((NM,volume 189))

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Abstract

Inherited visual disorders, major cause of visual impairment, can be subdivided into inherited retinal dystrophies (iRD), due to the progressive loss of photoreceptors and retinal pigment epithelium, or inherited optic neuropathies (iON), due to the loss of retinal ganglion cells whose axons form the optic nerve. IRDs and IONs represent two genetically and clinically heterogeneous groups for which cell replacement is one of the most encouraging therapeutic strategies.

For stem cell-based therapy, using human induced pluripotent stem cells (iPSCs) is crucial to obtain a relatively homogenous cell population to be transplanted. We will present effective strategies based on magnetic-activated cell sorting to select transplantable retinal ganglion cells or photoreceptors by targeting a cell surface antigen specifically expressed in each of the two retinal cell types from human iPSC-derived retinal organoids.

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Notes

  1. 1.

    Note that others separators may be more adapted to specific experiments, notably according to the number of organoids and cell number during isolation process

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Author information

Authors and Affiliations

  1. Institute de la Vision, Sorbonne Universite, INSERM, CNRS, Paris, France

    G. Orieux, O. Rabesandratana, G. Gagliardi & O. Goureau

Authors
  1. G. Orieux
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  2. O. Rabesandratana
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  3. G. Gagliardi
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  4. O. Goureau
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Corresponding author

Correspondence to O. Goureau .

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Editors and Affiliations

  1. Laboratory for Centrosome and Cytoskeleton Biology, Institute for Human Genetics, Heinrich-Heine-Universität, Universitätsklinikum Düsseldorf, Düsseldorf, Germany

    Jay Gopalakrishnan

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Orieux, G., Rabesandratana, O., Gagliardi, G., Goureau, O. (2023). Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting. In: Gopalakrishnan, J. (eds) Brain Organoid Research. Neuromethods, vol 189. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2720-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2720-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2719-8

  • Online ISBN: 978-1-0716-2720-4

  • eBook Packages: Springer Protocols

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