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Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection

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Cardiac Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2573))

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Abstract

Delivery of viral vectors to the heart represents a challenging endeavor. Besides vector design, the route of substrate administration is significantly influencing gene delivery success. The selective retrograde venous injection (SRVI) represents one of the most efficient percutaneous delivery strategies for transduction of the anterior left ventricular myocardium. In this chapter, we discuss the advantages and limitations of this vector delivery approach and provide a protocol for selective retrograde venous injection in a preclinical large animal model. As limited transgene expression frequently hampers generation of reliable proof-of-principle data and thus translation, this technique provides a valuable tool to ensure high myocardial transduction in preclinical research.

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Acknowledgments

This work is supported by grants from the Deutsche Forschungsgemeinschaft (RA 1668/1-1 and RA 1668/3-1) to P.W.R.

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Authors and Affiliations

  1. Department of Internal Medicine III, Cardiology, University Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany

    Philipp Schlegel & Philip W. J. Raake

  2. DZHK [German Centre for Cardiovascular Research], Partner Site, Heidelberg/Mannheim, Germany

    Philipp Schlegel & Philip W. J. Raake

  3. Department of Internal Medicine I, Cardiology, University Hospital Augsburg, University of Augsburg, Augsburg, Germany

    Philip W. J. Raake

Authors
  1. Philipp Schlegel
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  2. Philip W. J. Raake
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Corresponding author

Correspondence to Philip W. J. Raake .

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Editors and Affiliations

  1. Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Kiyotake Ishikawa

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Schlegel, P., Raake, P.W.J. (2022). Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 2573. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2707-5_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2707-5_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2706-8

  • Online ISBN: 978-1-0716-2707-5

  • eBook Packages: Springer Protocols

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