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Untargeted Metabolomics by Liquid Chromatography–Mass Spectrometry in Biomedical Research

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Mass Spectrometry for Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2571))

Abstract

Metabolomics, alone or in combination with other omics sciences, has shown great relevance in a large number of investigations in different branches of biomedicine, often providing novel discoveries and helping to expand the knowledge. Metabolomics analyses are carried out using different techniques, but in this chapter, we focus on liquid chromatography coupled to high-resolution mass spectrometry. The designated methodology consists of an untargeted approach for the analysis of plasma samples. The use of this method, with a reverse-phase column and electrospray ionization in positive mode, covers the detection of a broad range of metabolites, mainly of nonpolar and of intermediate polarity. This chapter also reviews the mass fragmentation spectra for the identification of bile acids, acylcarnitines, and glycerophospholipids.

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Acknowledgments

We would like to thank Fundación MEDINA for funding and Dr. José Pérez del Palacio for technical support.

Author information

Authors and Affiliations

  1. Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Granada, Andalucía, Spain

    Caridad Díaz

  2. Medical Oncology Unit, University Hospital of Jaén, Jaén, Andalucía, Spain

    Carmen González-Olmedo

Authors
  1. Caridad Díaz
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  2. Carmen González-Olmedo
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Corresponding author

Correspondence to Caridad Díaz .

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Editors and Affiliations

  1. Instituto de Investigación e Innovación Biomédica de Cádiz, Hospital Universitario Puerta del Mar, Cádiz, Spain

    Raúl González-Domínguez

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Díaz, C., González-Olmedo, C. (2023). Untargeted Metabolomics by Liquid Chromatography–Mass Spectrometry in Biomedical Research. In: González-Domínguez, R. (eds) Mass Spectrometry for Metabolomics. Methods in Molecular Biology, vol 2571. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2699-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2699-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2698-6

  • Online ISBN: 978-1-0716-2699-3

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