Abstract
Super-resolution microscopy methods enable the visualization of biological processes on the level of a few nanometers. However, the application of these techniques in biological systems is limited by the availability of small affinity reagents. Slow off-rate-modified aptamers as nucleic acid analogues to antibodies have been successfully applied to improve the resolution and quantification of DNA-PAINT. In this chapter, we describe a protocol for using SOMAmers as labeling reagents for super-resolution microscopy.
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References
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Strauss, S., Jungmann, R. (2023). Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT. In: Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2695-5_13
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DOI: https://doi.org/10.1007/978-1-0716-2695-5_13
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2694-8
Online ISBN: 978-1-0716-2695-5
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