Abstract
The nonhuman primate (NHP) animal model is an important predictive preclinical model for developing gene and cell therapies. It is also an experimental animal model used to study hematopoietic stem and progenitor cell (HSPC) biology, with the capability of serving as a step for the translation of the basic research concepts from small animals to humans. Lentiviral vectors are currently the standard gene delivery vehicles for transduction of HSPCs in the clinical setting. They have proven to be less genotoxic and more efficient than the previously used murine γ-retroviruses. Transplantation of lentiviral vector–transduced HSPCs into autologous macaques has been well developed over the past two decades. In this chapter, we provide detailed methodologies for lentiviral vector transduction of rhesus macaque HSPCs, including production and titration of lentiviral vector, purification of CD34+ HSPCs, and lentiviral vector transduction and assessment.
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Acknowledgments
We are grateful to Idalia Yabe, Xing Fan, and Stefan Cordes for their helpful discussions and critical reading and to Naoya Uchida, John Tisdale, Robert Donahue, So Gun Hong, Aylin Bonifacino, Stephanie Sellers, and many others within the laboratory and the primate facility for their contributions to development of rhesus macaque transplantation and gene transfer methodologies. We acknowledge funding from the NHLBI Division of Intramural Research.
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Wu, C., Hong, S.G., Bonifacino, A., Dunbar, C.E. (2023). Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells. In: Pelus, L.M., Hoggatt, J. (eds) Hematopoietic Stem Cells. Methods in Molecular Biology, vol 2567. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2679-5_5
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DOI: https://doi.org/10.1007/978-1-0716-2679-5_5
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