Abstract
Starch is important material in plant tissues, especially for storage tissues. Starches from different plant resources or tissues vary in morphology, content, and physicochemical properties. Starch and iodine can bind specifically to present the shapes and sizes of starch granules in plant tissues. Here, we describe some methods for staining starch in leaf, pollen grain, and starchy seeds with iodine solution. In addition, the isolated starch can also be stained with iodine solution to exhibit its shape and size.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Jane JL, Kasemsuwan T, Leas S, Zobel H, Robyt JF (1994) Anthology of starch granule morphology by scanning electron microscopy. Starch 46:121–129
Lindeboom N, Chang PR, Tyler RT (2004) Analytical, biochemical and physicochemical aspects of starch granule size, with emphasis on small granule starches: a review. Starch 56:89–99
Tetlow IJ, Bertoft E (2020) A review of starch biosynthesis in relation to the building block-backbone model. Int J Mol Sci 21:7011
Han H, Hou J, Yang N, Zhang Y, Chen H, Zhang Z et al (2019) Insight on the changes of cassava and potato starch granules during gelatinization. Int J Biol Macromol 126:37–43
Zhao L, Pan T, Guo D, Wei C (2018) A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed. Plant Methods 14:16
Qiu J, Ren Y, Zhao L, Zhang B, Wei C (2021) A simple dry sectioning method for obtaining whole-seed-sized resin section and its applications. J Vis Exp 167:1–13
Lin L, Guo D, Zhao L, Zhang X, Wang J, Zhang F et al (2016) Comparative structure of starches from high-amylose maize inbred lines and their hybrids. Food Hydrocoll 52:19–28
Acknowledgment
This work was supported by grants from the National Natural Science Foundation of China (32071927), the Talent Project of Yangzhou University, and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zhao, S., Ren, Y., Wei, C. (2023). Staining Starch with Iodine Solution. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_23
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2675-7_23
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2674-0
Online ISBN: 978-1-0716-2675-7
eBook Packages: Springer Protocols