Abstract
The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.
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Acknowledgment
This study was supported by a Discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) and by the Canada Research Chairs Program. Sample preparation and image acquisition were performed at the McGill University ECP3-Multi-Scale Imaging Facility, Sainte-Anne-de-Bellevue, Québec, Canada. We thank Dr. Jozef Mravec from the University of Copenhagen for providing us with COS488.
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Chebli, Y., Geitmann, A. (2023). Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_22
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DOI: https://doi.org/10.1007/978-1-0716-2675-7_22
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