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Localizing Molecules in Plant Cell Walls Using Fluorescence Microscopy

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2566))

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Abstract

Autofluorescence of plant tissues can be used as a label-free method to detect a range of phenolic-based cell wall components including lignin, suberin, and ferulate using widefield or confocal fluorescence microscopy. Likewise, fluorescently labeled antibodies can be used to localize specific carbohydrate molecules including arabinoxylan, β-1,4 galactan, glucomannan, glucuronoxylan, pectins, and xyloglucan. When combined, these two methods allow detailed study of topochemistry in different plant tissues for phenotyping of mutant varieties and plant biology studies. This article describes the protocols for fluorescent detection and imaging of molecules in plant cell walls using autofluorescence and immunofluorescence.

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Acknowledgments

This work was funded by Scion’s Strategic Science Investment Fund, Ministry of Business, Innovation and Employment, Wellington, New Zealand.

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Authors and Affiliations

  1. Scion Crown Research Institute, Rotorua, New Zealand

    Lloyd A. Donaldson

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  1. Lloyd A. Donaldson
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Correspondence to Lloyd A. Donaldson .

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Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, PAVIA, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, PAVIA, Italy

    Marco Biggiogera

  3. Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, VERONA, Italy

    Manuela Malatesta

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Donaldson, L.A. (2023). Localizing Molecules in Plant Cell Walls Using Fluorescence Microscopy. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_20

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  • DOI: https://doi.org/10.1007/978-1-0716-2675-7_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2674-0

  • Online ISBN: 978-1-0716-2675-7

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