Abstract
The chromatin dispersion test (CDT) is based on the removal of nuclear proteins under the assumption that cells with fragmented DNA produce a typical halo of circular DNA loops, which is absent in cells with non-fragmented DNA. This method represents a simple, rapid, accurate, highly reproducible, and inexpensive technique to assess nuclear DNA damage in somatic cells. The visualization of DNA damage and the capacity of the test to provide a threshold value to discriminate between high and low levels of cervical lesions would aid in determining the malignant transformation. All of these advantages associated with the CDT protocol could promote this technique as a tool for the quick and reliable diagnosis of cervical epithelial disorders, even at primary-care centers.
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Acknowledgments
We would like to thank Sanjuana L. Guardado-Limón for her excellent technical assistance.
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Cortés-Gutiérrez, E.I., Fernández, J.L., Dávila-Rodríguez, M.I., García de la Vega, C., Gosálvez, J. (2023). Chromatin Dispersion Test to Asses DNA Damage in Cervical Epithelial Cells. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_17
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DOI: https://doi.org/10.1007/978-1-0716-2675-7_17
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